BIOL 112 BIOL112 Photosyntheis Lab – Texas A&M
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BIOL 112 BIOL112 Photosyntheis Lab – Texas A&M
The Effects of Light Intensity, Light Wavelengths, and Absorption Spectrum on Photosynthesis
Abstract
Photosynthesis is the process in which eukaryotic cells utilized solar energy to convert the carbon dioxide in the air into carbohydrates. (1) Water and electrons are inputted in the process to yield the final products of glucose and oxygen.
This reaction is affected by the intensity of light, the light’s wavelength, and each pigment apart of the photosynthesis yield better results depending on the wavelength of light.
To test the reaction’s results in varying light intensity, the electron acceptor DPIP was measured (which in returns yields the results of the amount of photosynthesis taking place) in varying light treatments.
Description
BIOL 112 BIOL112 Photosyntheis Lab – Texas A&M
The Effects of Light Intensity, Light Wavelengths, and Absorption Spectrum on Photosynthesis
Abstract
Photosynthesis is the process in which eukaryotic cells utilized solar energy to convert the carbon dioxide in the air into carbohydrates. (1) Water and electrons are inputted in the process to yield the final products of glucose and oxygen.
This reaction is affected by the intensity of light, the light’s wavelength, and each pigment apart of the photosynthesis yield better results depending on the wavelength of light.
To test the reaction’s results in varying light intensity, the electron acceptor DPIP was measured (which in returns yields the results of the amount of photosynthesis taking place) in varying light treatments.
By measuring the absorbance(as it decreased, more DPIP was used (turning from the color blue to clear), more photosynthesis was occurring).
It was observed that the optimal light distance for photosynthesis was 30 cm as 24 cm away from the light source was too close and bright for optimal photosynthetic activity.
While 49 cm was too far from the test tube to catch the light emitted by the lamp. In the light wavelength experiment, the color of the lights was changed (i.e. Red, Blue, Green, White, and Dark) to show which wavelength provides for the fastest photosynthesis.
Spinach leaves when accumulating oxygen (a byproduct of photosynthesis) were allowed to float to the surface as a means for measuring photosynthetic activity.
It was seen that blue light (other than the positive white light control) allowed for best photosynthetic ability as green light is often reflected by chlorophyll (main pigment responsible for photosynthesis).
Lastly, in the absorption spectrum experiment, the pigments, Carotene, Chlorophyll a, Xanthophyll, and Chlorophyll b were tested for absorption at different wavelengths.
It was seen that Chlorophyll b, Carotene, and Xanthophyll have best absorption around wavelength 400 – 500 nm while Chlorophyll a peaks in400- 500 nm and 600 – 700 nm showing that green light is the least optimal light for photosynthesis to occur.
These experiments all portrayed the different factors that could affect photosynthesis: light intensity, wavelength, and absorption spectrum.
Introduction
For the light intensity experiment, the effect on photosynthesis depending on the changing light intensities was tested. Thylakoid membranes inside chloroplasts have pigments with Photosystem I’s optimal light wavelength being 700 nm and Photosystem II’s 680 nm.
The solar energy absorbed by these excite the electrons into a higher energy. By substituting NADPH,the electron carrier for photosynthesis, with DPIP the rate of photosynthesis can be observed. DPIP is originally blue, and when reduced to DPIPH2 it becomes clear.
The more reductions of DPIP, the clearer the solution, the less the absorption, and the more the reaction is taking place. Itwas seen to observe how light intensity from a distance of 24cm, 30cm, and 49 cm, the rate of photosynthesis would occur. One tube was left in the dark for a control.
BIOL 112 BIOL112 Photosyntheis Lab – Texas A&M
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BIOL 112 BIOL112 Photosyntheis Lab – Texas A&M
BIOL 112 – Spring 2013 Register Now BIOL Lab 2 and 3 Questions Only.docx Textbook Exercises Campbell Biology image Campbell Biology Ch 1, Section 1.1 The Study of life reveals unifying themes., Exercise F1.4
Eukaryotes are larger and more complex than prokaryotes.Campbell Biology Ch 2, Section 2.1 Matter consists of chemical elements in pure form and in combinations called compunds, Exercise 1Table salt (NaCl) is a compound made of the elements sodium and chlorine.Campbell Biology Ch 3, Section 3.1
Polar covalent bonds in water molecules result in hydrogen bonding, Exercise F3.2Oxygen has greater electronegativity than hydrogen and it tends to pull shared electrons in a water…Campbell Biology Ch 4, Section 4.1 Organic chemistry is the study of carbon compunds, Exercise F4.2
An increase in the concentration of reactants affects the concentration of products.Campbell Biology Ch 5, Section 5.1 Macromolecules are polymers, built from monomers, Exercise 1The main classes of biological molecules perform crucial biological functions ranging from fuel to…Campbell Biology Ch 6, Section 6.1
Biologists use microscopes and biochemistry to study cells, Exercise F6.3With a change in the cutting plane, different internal regions will be exposed.Campbell Biology Ch 7, Section 7.1 Cellular membranes are fluid mosaics of lipids and proteins, Exercise F7.2
BIOL 112 BIOL112 Photosyntheis Lab – Texas A&M
The region that contains the phosphate group (in glycerol) is in contact with water.Campbell Biology Ch 8, Section 8.1 An organism’s metabolism transforms matter and energy, subject to the laws of thermodynamics, Exercise 1Passive transport mechanisms are thermodynamically favorable.Campbell Biology Ch 9, Section 9.1
Catabolic pathways yield energy by oxidizing organic fuels, Exercise F9.4The electrons present in the nicotinamide molecule determine the oxidized and reduced forms.Campbell Biology BIO LAB AND LECTURE Tests Questions & Answers Showing 1 to 8 of 12 View all Please refer to the attachment to answer this question.
This question was created from Deuterostomes_protocolanddatasheet_QV (1).docx. 1 2 3 4 5 Please refer to the attachment to answer this question. This question was created from Deuterostomes_protocolanddatasheet_QV (1).docx. 1 2 3 4 5
Which of the following is NOT a major difference between "Bryophytes" and ALL other land plant phyla? absence vs. presence of xylem and phloem 1 2 3 4 5 An individual fern sporophyte is able to produce far more spores than an individual moss sporophyte because, unlike the moss, the fern sporophyte has 1 2 3 4 5
BIOL 112 BIOL112 Photosyntheis Lab – Texas A&M
Which of the following is NOT evidence supporting the hypothesis of the endosymbiotic evolution of mitochondria and chloroplasts? photosynthetic eukaryotes 1 2 3 4 5 Mutations that cause changes in development are thought to be important in the appearance of "evolutionary novelty" because ontogeny recapitulates 1 2 3 4 5
Which of the following is NOT considered to be an important correlate of the oxygen revolution? the accumulation of ozone screened out harmful ultraviolet 1 2 3 4 5 Most modern reptiles are grouped in the Order Squamata. The ancestors of these reptiles originated in the Mesozoic and gave rise to numerous and varied species 1 2 3 4 5
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